PRODUCTIVITY INDICES IN ROTIFER BRACHIONUS PLICATILIS FEEDING ON MICROALGAE OF DIFFERENT TAXONOMIC GROUPS
Keywords:
microalgae, rotifers, Brachionus plicatilis, flow cytometry, productivity, ration, clearance rate, gross growth efficiency, Dunaliella, Isochrysis, PhaeodactylumAbstract
Major productive indices (the rates of production, grazing and clearance; specific growth rate; gross growth efficiency) were estimated experimentally in batch cultures of the parthenogenetic strain of Brachionus plicatilis fed by Dunaliella salina, Isochrysis galbana and Phaeodactylum tricornutum. The highest rotifer numbers (320 ind ml-1) their specific growth rates (1.4 day-1) and gross growth efficiency (K1 = 27.6 %) were observed when they were fed by P. tricornutum. The rotifer clearance rate was lowest (1.4 μl ind-1 h-1), providing an indirect evidence that the rotifers made less efforts on locating and catching P. tricornutum. Feeding on I. galbana demanded higher clearance rate (2.3 μl ind-1 h-1) and ration (0.17 μg WW ind-1 h-1) but resulted in much lower K1 (11.3%). Thus, P. tricornutum proved to be the best live food for B. plicatilis. Bacteria introduced to the experimental medium with non-axenic microalgal cultures were actively grazed by rotifers. The bacterial ration of the rotifers (1.5 to 4 ng WW ind-1 h-1) was probably underestimated if the bacterial production in the test flasks was sufficiently higher than in the control ones (with no grazers).
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