FEATURES OF SECONDARY CAROTENOGENESIS IN VEGETATIVE CELLS OF HAEMATOCOCCUS PLUVIALIS FLOTOW (CHLOROPHYСEAE) UNDER DIFFERENT CONDITIONS OF MINERAL SUPPLY

Authors

  • N. V. Terentieva
  • G. S. Minyuk
  • I. V. Drobetskaya
  • I. N. Chubchikova

Keywords:

Haematococcus pluvialis, cultivation, astaxanthin, nitrogen, phosphorus

Abstract

Influence of nitrogen and phosphorus concentrations in nutrient medium on growth rate and astaxanthin accumulation in vegetative cells of green microalga Haematococcus pluvialis under condition of single-stage semi-continuous culture has been investigated. It has been shown that the maintenance of minimum level of biogenic elements in a medium within the limits of 0.2 – 0.4 mM N and 0.01 – 0.02 mM P is necessary for keeping of monad cell structure, long-term cell division and astaxanthin accumulation.

References

Методы гидрохимических исследований основных биогенных элементов. – М.: ВНИРО, 1988. – 119 с.

Минюк Г. С., Дробецкая И. В., Чубчикова И. Н., Терентьева Н. В. Исследование вторичного каротиногенеза у одноклеточных водорослей // Проблемы биологической океанографии XXI века: Тез. докл. междунар. науч. конф., посвященной 135-летию ИнБЮМ, Севастополь, 19 – 21 сент. 2006 г. – Севастополь: ЭКОСИ–Гидрофизика, 2006. – С. 154.

Минюк Г. С., Терентьева Н. В., Дробецкая И. В. Сравнительная характеристика морфологических и физиолого-биохимических признаков трех штаммов Haematococcus pluvialis Flotow (Chlorophyta, Chlamydomonadales) // Альгология. – 2007. – 17, № 2. – С. 148 – 159.

Boussiba S. Carotenogenesis in the green alga Haematococcus pluvialis: сellular physiology and stress response // Physiol. Plant. – 2000. – 108. – P. 111 – 117.

Brinda B. R., Sarada R., Kamath B. S. et al. Accumulation of astaxanthin in flagellated cells of Haematococcus pluvialis – cultural and regulatory aspects // Curr. Sci. – 2004. – 87. – N, 9. – P 1290 - 1295.

Del Campo J, A., García-González M., Guerrero M. G. Outdoor cultivation of microalgae for carotenoid production: current state and perspectives // Appl. Microbiol. Biotechnol. – 2007. – 74, N.6. – P. 1163 – 1174.

Del Rio E., Acien F.G., Garcia-Malea M.C. et al. Efficient one-step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture // Biotechnol. Bioeng. – 2005. – 91, N 7. – P. 808 – 815.

Dominguez-Bocanegra A. R., Legarreta I. G. et al. Influence of environmental and nutritional factors in the production of astaxanthin from Haematococcus pluvialis // Biores. Technol. – 2004. – 92, N. 2. – P. 209 – 214.

Eonseon J., Lee C.-G., Polle J. E .W. Secondary carotenoid accumulation in Haematococcus (Chlorophyceae): biosynthesis, regulation and biotechnology // J. Microbial. Biotechnol. – 2006. – 16, N. 6. – P. 821 – 831.

Fábregas J., Otero A., Maseda A et al. Domíguez A. Two-stage cultures for production of astaxanthin from Haematococcus pluvialis // J. Biotechnol. – 2001. – 89. – P. 65 – 71.

Hagen C., Grünewald K., Xyländer M. et al. Effect of cultivation parameters on growth and pigment biosynhesis in flagellated cells of Haematococcus pluvialis // J. Appl. Phycol. – 2001. – 13. – P. 79 - 87.

Kobayashi M., Kakizono T., Nagai S. Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst cells of green unicellular alga Haematococcus pluvialis // Appl. Environ. Microbiol. – 1993. – 59, N 3 – P. 867 – 873.

Lee Y.K., Ding S. Y. Cell cycle and accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta) // J. Phycol. – 1994. – 30. – P. 445 – 449.

Liaaen-Jensen S., Andrewes A. G. Analyses of carotenoids and related polyene pigments // Meth. Microbiol. – 1985. – 18. – P. 235 - 255.

Syrett P. J. Nitrogen metabolism of microalgae // Can. Bull. Fish. Aquat. Sci. - 1981. – 210. – P. 182 – 210.

Yamasaki H. Nitrite-dependent nitric oxide production pathway: implications for involvement of active nitrogen species in photoinhibition in vivo // Phil. Trans. R. Soc. Lond. – 2000. – 355. – P. 1477 - 1488

Published

2023-05-26