CONTENTS AND UPTAKE OF INORGANIC NITROGEN IN THE BLACK SEA
Keywords:
nitrate, ammonium, uptake rates, half-saturation constant, Black SeaAbstract
Relations between concentrations of inorganic nitrogen (nitrate and ammonium) in the water and their uptake rates by phytoplankton in the Black Sea during the different seasons were analyzed based on data of nitrate and ammonium consumption. It is shown that this relation is described by the curve of saturation type, and its parameters change in accordance with the seasonal dynamics of hydrochemical conditions. Saturation constants calculated for the processes of nitrate and ammonium uptake are near to the monthly average concentrations of inorganic nitrogen in the surface layer. On the average, nitrate and ammonium uptake rates achieves a half from its maximal value. Thus dissolved inorganic nitrogen concentration limits rates of their consumption only in two times. Summer phytoplankton takes up inorganic nitrogen more effectively under their low concentration in the environment as compared to a winter period.
References
Ведерников В. И. Особенности распределения первичной продукции и хлорофилла в Чёрном море в весенний и летний периоды // Изменчивость экосистемы Чёрного моря: естественные и антропогенные факторы. – М.: Наука, 1991. – С. 128 – 147.
Ведерников В. И., Сергеева О. М., Коновалов Б. В. Экспериментальное изучение зависимости скорости роста и фотосинтеза фитопланктона Чёрного моря от условий минерального питания // Экосистемы пелагиали Чёрного моря. – М.: Наука, 1980. – С. 140 – 157.
Виноградов В. Е., Сапожников В. В., Шушкина Э. А. Экосистемы Чёрного моря. – М.: Наука, 1992. – 112 с.
Кирикова М. В. Поглощение неорганического фосфора микропланктоном и распределение биогенных элементов в Чёрном море и Северной Атлантике: дисс… канд. биол. наук: 03.00.18. - Севастополь, 1989. – 156 с.
Кирикова М. В., Кривенко О. В. Неорганические формы азота и кремния в Чёрном море в весенний период // Экология моря. – 2001. – Вып. 56. – С. 18 – 22.
Кривенко О. В. Динамика потребления неорганических соединений азота микропланктоном в Чёрном море: Автореф. дисс… канд. биол. наук: 03.00.17 / ИнБЮМ НАНУ. – Севастополь, 2005.
Кривенко О. В., Лукьянова А. И. Потребление минерального азота микропланктоном Чёрного моря в связи с гидрохимическими условиями // Океанология. – 1994. – 34, №2. - C. 232 - 239.
Сапожников В. В. Новые представления о гидрохимической структуре Чёрного моря // Изменчивость экосистемы Чёрного моря: естественные и антропогенные факторы. – М.: Наука, 1991. – С. 34 – 46.
Basturk O., Tugrul S., Konovalov S. et al. Variations in the vertical structure of water chemistry within the three hydrodynamically different regions of the Black Sea // Sensitivity to change: Black Sea, Baltic Sea and North Sea. - Series 2: Environment. – Dordrecht: Kluwer Acad. Publ., 1997. - 27. - P.183 –196.
Boyd P. W., Harrison P. J. Phytoplankton dynamics in the NE subarctic Pacific // Deep-Sea Res. II. – 1999. – 46. – P. 2405 – 2432.
Boyd P. W., Law C. S., Wong C. S. et al. The decline and fate of an iron-induced subarctic phytoplankton bloom // Nature. – 2004. – 428. – P. 549 – 553.
Boyd P. W., Watson A. J., Law C. S. et al. Amesoscale phytoplankton bloom in the polar Southern Ocean stimulated by iron fertilization // Nature. –2000. – 407. – P. 695–702.
Button D. K. On the theory of control of microbialgrowth kinetics by limiting nutrient concentration // Deep-Sea Res. – 1978. – 25. – P. 1163 – 1177.
Button D. K. Affinity of organisms for substrate // Limnol. Oceanogr. – 1986. – 31. – P. 453 – 456.
Caperon J., Meyer J. Nitrogen-limited growth of marine phytoplankton. II. Uptake kinetics and their role in nutrient limited growth of phytoplankton // Deep-Sea Res. – 1972. – 19, No 9. – P. 619 – 632.
Carpenter E. J., Guillard R. R. Intraspecific differences in nitrate half-saturation constants for three species of marine phytoplankton // Ecology. – 1971. – 52. – P. 183 – 185.
Cochlan W. P., Harrison P. J. Kinetics of nitrogen (nitrate, ammonium and urea) uptake by the pico-flagellate Micromonas pusilla (Prasinophyceae) // J. Exp. Mar. Biol. Ecol. – 1991. – 153. – P. 129 – 141.
Conley D. J., Malone T. C. Annual cycle of dissolved silicate in Chesapeake Bay: implications for the production and fat of phytoplankton biomass // Mar. Ecol. Prog. Ser. – 1992. – 81. – P. 121 – 128.
Dodds W. K., Strausss E. A., Lehmann R. Nutrientdilution and removal bioassays to estimate phyto-1. plankton response to nutrient control // Arch. Hydrobiol.
– 1993. – 128. – P. 467 – 481.
Dortch Q. The interaction between ammonium and nitrate uptake in phytoplankton // Mar. Ecol. Prog. Ser. – 1990. – 61. – P. 183 – 201.
Droop M. R. Vitamin B12 and marine ecology IV. Kinetics of uptake, growth and inhibition in Mono-chrysis lutheri. // J. Mar. Biol. Assoc.UK. – 1968. – 48. – P. 689 – 733.
Dugdale R. C. Nutrient limitation in the sea: dynamics, identification and significance // Limnol. Oceanogr. – 1967. – 12. – P. 685 – 695.
Dugdale R. C., Goering J. J. Uptake of new and regenerated forms of nitrogen in primary productivity // Limnol. Oceanogr. – 1967. – 12, No1. – P. 196 – 206.
Egge J. K., Aksnes D. L. Silicate as regulating nutrient in phytoplankton competition // Mar. Ecol. Prog. Ser. – 1992. – 83. – P. 281 – 289.
Elskens M., Baeyens W., Goeyens L. Contribution of nitrate to the uptake of nitrogen by phytoplankton in an ocean margin environment // Hydrobiologia. – 1997. – 353, No1 – 3. – P. 139 - 152.
Eppley R. W., Coatsworth J. L. Nitrate and nitrite uptake by Ditylum brightwellii. Kinetics and mechanisms // J. Phycol. – 1968. – 4. – P. 151 – 156.
Esler J. J., Marzolf E. R., Goldman C. R. Phosphorus and nitrogen limitation of phytoplankton growth in freshwaters of North America: A review and critique of experimental enrichments // Can. J. Fish. Aquat. Sci. – 1990. – 47. – P. 1468 – 1477.
Flynn K. J. A mechanistic model for describing dynamic multi-nutrient, light, temperature interactions in phytoplankton // J. Plankton Res. – 2001. – 23. – P. 977 – 997.
Flynn K. J. The determination of nitrogen status in microalgae // Mar. Ecol. Progr. Ser. – 1990. – 61. – P. 297 - 307.
Gervais F., Riebesell U., Gorbunoc M. Y. Changes in primary productivity and chlorophyll a in response to iron fertilisation in the Southern Polar Frontal Zone // Limnol. Oceanogr. – 2002. – 47. – P. 1324 – 1335.
Glibert P. M, McCarthy J. J. Uptake and assimilation of ammonium and nitrate by phytoplankton: indices of nutritional status for natural assemblages // J. Pl. Res. – 1984. – 6, No4. – P. 677 - 697.
Goldman J. C., Glibert P. M. Kinetics of inorganic nitrogen uptake by phytoplankton // Nitrogen in the marine environment. - New York: Academic Press, 1983. – P. 233 – 274.
Harrison P. J., Parslow J. S., Conway H. L. Determination of nutrient uptake kinetic parameters: acomparison of methods // Mar. Ecol. Prog. Ser. –1989. – 52. – P. 301 – 312.
Harrison W. G., Harris L. R., Irwin B. D. Nitrate and ammonium interactions at nanomolar concen-trations // Limnol. Oceanogr. – 1996. – 41, No1. – P. 16 – 32.
Healey F. P. Slope of the Monod equation as an indicator of advantage in nutrient competition // Microb. Ecol. – 1980. – 5. – P. 281 – 286.
Hecky R. E., Kilham P. Nutrient limitation of phytoplankton in freshwater and marine environments: a review of recent evidence on the effects of enrichment // Limnol. Oceanogr. – 1988. – 33. – P. 796 – 822.
Krivenko O. V., Burlakova Z. P., Eremeeva L. V. Basic characteristics of biotic nitrogen cycle in the open western part of the Black Sea// Ecosystem modelling as a management tool for the Black Sea. – Dordrecht: Kluwer Acad. Publ., 1998. – 1. – P. 121 – 136.
MacIsaac J. J., Dugdale R. C. The kinetics of nitrate and ammonia uptake by natural populations of marine phytoplankton // Deep-Sea Res. – 1969. – 16, No1. – P. 45 –57.
McCarthy J. J. The kinetics of nutrient utilization // Can. Bull. Fish. Aquat. Sci. – 1981. – 210. – P. 211 – 233.
Monod J. Recherches sur la croissance des cultures bacteriennes. Actualites scientifiques et industrielles. // Annu. Rev. Microbiol. – 1942. – 3. – P. 3 – 71.
Muggli D. L., Smith W. O. Regulation of nitrate and ammonium uptake in the Greenland Sea // Mar. Biol. – 1993. – 115. – P. 199 – 208.
Parslow J. S., Harrison P. J., Thomson P. A. Interpreting rapid changes in uptake kinetics in the marine diatom Thalassiosira pseudonana (Hustedt) // J. Exp. Mar. Biol. Ecol. – 1985. – 89. – P. 53 – 64.
Reay D. S., Nedwell D. B. Priddle J. et al. Temperature dependence of inorganic nitrogen uptake: Reduced affinity for nitrate at suboptimal temperatures in both algae and bacteria // Appl. Environ. Microbiol. – 1999. – 65, No6. – P. 2577 – 2584.
Stelmakh L. V., Krivenko O. V. Seasonal variability of "new" primary production in the western open part of the Black Sea // Oceanography of eastern Mediterranean and Black Sea: similarities and differences of two interconnected basins. - Ankara: Tübitak, 2003. - P. 471 – 478.
Yayla M. K., Yılmaz A., Morkoc E. The dynamics of nutrient enrichment and primary production related to the recent changes in the ecosystem of the Black Sea // Aquatic Ecosystem Health and Management. – 2001. – 4, No1. – P. 31 – 49.
Yılmaz A., Coban-Yıldız Y., Telli-Karakoc F. et al. Surface and midwater sources of organic carbon by photoautotrophic and chemoautotrophic production in the Black Sea // Deep-Sea Res. – 2006. – 53. – P. 1988 – 2004.
Yılmaz, A., Tugrul S., Polat C. et al. On the production, elemental composition (C, N, P) and distribution of photoautotrophic organic matter in the southern Black Sea // Hydrobiologia. – 1998. – 363. – P. 141 – 156.