CHARACTERISTICS OF TISSUE METABOLISM REORGANIZATION IN BIVALVE MOLLUSK ANADARA INAEQUIVALVIS (BRUGUIERE, 1789) UNDER EXPERIMENTAL STARVATION CONDITIONS
Keywords:
starvation, Anadara inaequivalvis (Bruguiere, 1789), tissue metabolism, protein, amino acids, glucose, enzyme activityAbstract
The effect of starvation on the orientation of metabolic processes in bivalve mollusk Anadara inaequivalvis (Black sea) was investigated under experimental conditions. The experiment lasted up to 18 days. Сhanges in the contents of some protein (protein, amino acids, urea) and carbohydrate (glucose, lactate, pyruvate) substrates and metabolites, and also the activity of six enzymes (lactate and malate dehydrogenase, alanine and aspartate transaminases, γ-glutamyl transferase, katepsin D) in foot, gills and hepatopancreas of the mollusk were investigated. It was shown that during initial stages of starvation (6 day) A. inaequivalvis used a resource of tissue lactate in a direction of oxidative decarboxylation reactions. Significant increase in activity of lactate dehydrogenase on a background of lactate content reduction and rise in pyruvate level in tissues was determined. The process of A. inaequivalvis adaptation to starvation involves using the amino acids reserves during tissue biosynthesis. It reflects the reduction of amino acids level in all studied tissues and rise in protein contents in the absence of pronounced changes in urea concentration in mollusk organ. Using of amino acids as an energy source for tissue during starvation occurs in the form fumarate reductase and succinate thiokinase reactions which allow to obtain additionally glycolytic metabolites. Increase in activities of alanine and aspartate transaminases in some confirm that. Hepatopancreas is found to be a donor of amino acids. In contrast to other tissues, food deprivation causes significant rise in katepsin D activity in hepatopancreas.
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