SCREENING OF GREEN MICROALGAE AS A POTENTIAL SOURCE OF NATURAL KETOCAROTENOIDS 2. FEATURES OF GROWTH AND SECONDARY CAROTENOGENESIS IN THE REPRESENTATIVES OF THE GENUS BACTEACOCCUS (CLOROPHYСEAE)

Authors

  • I. N. Chubchikova
  • I. V. Drobetskaya
  • G. S. Minyuk
  • N. V. Dantsyuk
  • E. S. Chelebiyeva

Keywords:

Bracteacoccus spp., cultivation, astaxanthin, canthaxanthin

Abstract

Productivity characteristics of two Bracteacoccus species (B. minor и B. giganteus) under screening of commercially suitable natural ketocarotenoid producers by uniform mode of two stage batch cultures have been determined for the first time. It has been shown that in accordance with to specific growth rate (0.50 – 0.59 day-1), total carotenoid yield (≈ 1.7 mg·l-1·day-1) and carotenoidcontent in dry biomass (0.9 – 1.0 %) both species can be recommended for further studies on developing of their mass culture technology.

References

Костіков І. Ю., Романенко П. О., Демченко Е. М. і інш. Водорості грунтів України (історія та методи дослідження, система, конспект флори). – Київ: Фітосоціоцентр, 2001. – 300 с.

Минюк Г. С., Дробецкая И. В., Чубчикова И. Н., Данцюк Н. В., Челебиева Э. С. Скрининг зелѐных микроводорослей как потенциальных источников природных кетокаротиноидов. Актуальность, стратегия и тактика исследований // Экология моря. – 2010. – Вып. 80. – С. 67 – 78.

Терентьева Н. В., Дробецкая И. В., Чубчикова И. Н. и др. Влияние освещенности на физиолого-биохимические характеристики зеленой микроводоросли Haematococcus pluvialis (Chlamydomonadales) // Экология моря. – 2008. – Вып. 75. – С. 82 – 88.

Урмыч Е.М., Бердыкулов Х.А., Эшпулатова М.Б. Продуктивность микроводорослей в интенсивных условиях культивирования // Альгология. – 2008. – 18, No 3. – С. 347-352.

Чубчикова И. Н., Минюк Г. С., Дробецкая И. В. и др. Хлорококковые микроводоросли как потенциальный источник природных кетокаротиноидов // Экология моря – 2009. – Вып. 77. – С. 77 – 83.

Abe K., Hattori H., Hirano M. Accumulation and antioxidant activity of secondary carotenoids in the aerial microalga Coelastrella striolata var. multistriata // Food Chem. – 2007. – 100, № 2. – P. 656-661.

Aquasearch Technical report 1002.001. Analysis of total astaxanthin in algae meal prepared from Haematococcus pluvialis // – Aquasearch. Inc., http://www.fda.gov.

Chapman D. J. Formation and analysis of secondary carotenoids // Experimental phycology. A laboratory manual. – Cambridge Univer. Press, 1988. – P. 104 – 110.

Czygan F.-C. Primäre und secundäre Carotenoide in chlorokokkalen Algen // Arh. Hydrobiol. Suppl. – 1982. – 60, No 4. – P. 470 – 488.

Del Campo A., Rodrigues H., Moreno J. et al. Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta) // Appl. Microbiol. Biotechnol. – 2004. – 64, No 6. – P. 848 – 854.

Deming D. M., Boileau T. W.-M., Heintz K. H. et al. Carotenoids: Linking Chemistry, Absorption and Metabolism to Potential Roles in Human Health and Disease// Handbook of Antioxidants. – New York-Basel: Marcel Dekker, Inc., 2002 – P.178 –211.

Fujii K., Imazato E., Nakashima H. et al. Isolation of non fastidious microalgae with astaxanthin-accumulation property and its potential for application to aquaculture // Aquaculture. – 2006. – 261. – P. 285 – 293.

Jeffrey S. W., Mantoura R. F. C., Bjørnland T. Data for identification of 47 key phytoplankton pigments / Phytoplankton pigments in oceanography: guidelines to modern methods. – UNESCO Publishing: Paris, 1997. – P. 493 – 553.

Kopecký J., Schoefs B., Loest K. et al. Microalgae as source for secondary carotenoid production: a screening study // Algological. Studies. – 2000. – 98. – P. 153 – 168.

Lewis L. A. Diversity and phylogenetic placement of Bracteacoccus Tereg (Chlorophyceae, Chlorophyta) based on 18S ribosomal RNA gene sequence data // J. Phycol. – 1997. – 33, No 2. – P. 279 –285.

Liu B-H., Lee Y-K. Composition and biosynthesis pathways of carotenoids in the astaxanthin-produsing green alga Chlorococcum sp. // Biotechnol. Lett. – 1999. – 21. – P. 1007 –1010.

Orosa M., Torres E., Fidalgo P. et al. Production and analysis of secondary carotenoids in green algae // J. Appl. Phycol. – 2000. – 12, No. 3/5. – P.553 – 556.

Qin S., Liu G.-X., Hu Z.-Y. The accumulation and metabolism of astaxanthin in Scenedesmus obliquus (Chlorophyceae) // J. Process biochemistry. – 2008.– 43, No 8. – P. 795 – 802.

Rodriguez-Amaya D. B. A guide to carotenoid analysis in foods / Washington: ILSI Press, 2001. –71 p.

Wellburn A. R. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution // J. Plant Physiol. – 1994. – 144. – P.307 – 313.

Published

2023-05-20