FLOW CYTOMETRY FOR THE ASSESSMENT OF PHYSIOLOGICAL ACTIVE CELLS IN BATCH CULTURES OF PHAEODACTYLUM TRICORNUTUM ТА NITZSCHIA SP.
Keywords:
Viability of microalgae, flow cytometry, fluorescein diacetate, enzyme activity, enzyme esteraseAbstract
Dynamics of the ratio between physiologically active (viable) and inactive (dead) cells was studied in monospecific batch cultures of the diatoms Phaeodactylum tricornutum and Nitzschia sp., using flow cytometry. Over the period of culture senescence, intracellular chlorophyll content and enzyme activity decreased while the number of dead cells increased. The cultures of Nitzschia sp. and P. tricornutum achieved the stationary phase in 4 and 15 days, respectively. In the exponential growth phase, contribution of dead cells to the total abundance did not exceed 10% in both the cultures while in the stationary phase, it rised up to 70 % in P. tricornutum and almost 100 % in Nitzschia sp. The approach approved can be successfully applied for estimating viable cell abundances in natural populations of the species.
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