EFFECT OF REVERSE RESTORATION OF METHYLENE BLUE COLOURING AND OPPORTUNITY OF ITS APPLICATION FOR ESTIMATION OF FUNCTIONAL ABILITY OF BACTERIAL COMMUNITY
Keywords:
methylene blue, dehydrogenase activity, bacteriobethos, Black SeaAbstract
The effect of increase of optical density of methylene blue at contact of samples with oxygen of air after anaerobic expositions, was used by us for researches dehydrogenase activity . The opportunity of its application for an estimation of functional activity of mycrobial populations is considered.
References
Горбенко Ю. А. О наиболее благоприятном количестве «сухого питательного агара» в средах для культивирования морских гетеротрофных микроорганизмов // Микробиология. – 1961. – 30, 1. – С. 168 – 172.
Готтшалк Г. Метаболизм бактерий. – М.: Мир, 1982. – 310 с.
Мецлер Д. Биохимия: в 3 т. / Мир. – М., 1980. – Т. 2: Химические реакции в живой клетке. – 606 с.
Пирс Э. Гистохимия. – М.: Изд-во иностр. лит-ры, 1962. – 962 с.
Чекалов В. П. Определение с помощью метиленового синего сорбционной способности и дегидрогеназной активности донных отложений // Экология моря. – 2006. – Вып. 72. – С. 103-109.
Burtton G. A., Lanza G. R. Variables affecting two electron transport system assays // Appl. Environ. Microbiol. – 1986. – 51. – P. 931 – 937.
Gong P. Dehydrogenase activity in soil: a compari-son between the TTC and INT assay under their optimum conditions // Soil Biol. Biochem. – 1997. – 29. – P. 211 – 214.
Lenhard G. The dehydrogenase activity in soil as measure of the activity of soil microorganisms // Z. Pflanzenernaehr Dueng. Bodenked. – 1956. – 73. – P. 1 – 11.
Mathew M., Obbard J. P. Optimization of the dehydrogenase assay for measurement of indigenous microbial activity in beach sediments contaminated with petroleum // Biotechnol. Lett. – 2001. – 23. – P. 227 – 230.
Matson E. A. Community electron transport of prokaryotes in euryoxic estuarine sediment // Mar. Ecol. Prog. Ser. – 1987. – 41. – P. 71 – 78.
Ross D. J. Some factors influencing the estimation of dehydrogenase activities of some soils under pasture // Soil Biol. Biochem. – 1971. – 3. – P. 97 – 110.
